FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405366

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405366
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16105532
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG28600.016.4036521
GTATTAA43550.015.9980371
GTACTAA29700.015.5603461
GTATATA46950.015.4360631
GTCCTAG37650.015.1573891
GTACTAG16900.014.9158351
GTGCTAG37300.014.7364421
GTCTTAG32550.014.628161
GTATAGG25700.014.1677871
GCTCTAG45400.014.0351581
GTCCTAA30200.013.9115691
TCTAGAC34300.013.77988153
GTTCTAG41750.013.5850211
CTAGACT35250.013.1106234
GCCCTAG37350.012.9357091
CTACTAG22000.012.8903321
GTATTAT41350.012.8697411
TCTATAC33600.012.8165653
GGATTAG31700.012.8115151
GTATATG41650.012.6929851
Produced by FastQC (version 0.11.5)