FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405367

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405367
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16105532
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG18850.020.090771
GTACTAA28700.017.4718271
GTATTAG30200.016.1395761
GTCCTAG37450.015.3559351
GTTCTAA42450.015.0341181
GGTCTAA29950.014.869321
GTATTAA42950.014.7774541
GTCTTAA39950.014.658311
GTCCTAA32100.014.3103571
GTTCTAG42700.014.2070021
CTAAGCG10600.014.19964
GTATTAT40150.014.1486071
GTATATA47000.014.1009441
GTGCTAG35250.014.0263361
CGAATCG7600.013.8149639
GTCTTAG33950.013.6338461
GCTCTAG44350.013.28311351
GTGCTAA35800.012.8313541
GTACATA39800.012.6871381
CTATACT33150.012.671014
Produced by FastQC (version 0.11.5)