FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405368

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405368
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15964361
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAA40550.016.7460921
GTCCTAG35700.016.6679951
GTATTAG27000.015.6864351
GTACTAG17500.015.6012421
GTACTAA27600.015.0917821
GTATATA46650.014.7064231
ATTAGAC30100.014.4200243
TCTATAC29100.014.1938373
GTGTTAG33900.014.1456691
GTCTATA26300.013.9744971
GTTCTAG42850.013.8867531
GTATAAG29800.013.8601641
GTCCTAA31600.013.7352711
GTATAGG23200.013.7295431
GTATAGA32500.013.6780121
CTATACT33200.013.38999754
GTACAGG72900.013.2040681
GTGCTAG34450.013.1069951
GTCTTAG32950.013.0662911
CCTCTAG63550.013.0537541
Produced by FastQC (version 0.11.5)