FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405371

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405371
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15895791
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCTAG39250.017.042941
GTACTAG17900.016.6309381
GTCCTAA30150.016.2626571
GTATTAG27450.016.0760381
GTTCTAG43550.014.7168111
GTGCTAG36400.014.5286871
GTATAGG23200.014.4921911
GTCTTAG33200.014.3466881
GCTCTAG45400.013.9628321
GTTCTAA43200.013.6199761
GTGTTAG35400.013.554021
GGTCTAG30300.013.5236531
GTATATG42350.013.4798491
GTATTAA41200.013.4310721
TCTATAC30600.013.2668433
CTATACT33900.013.2142534
GTACAGG69500.013.2028371
GTATATA45050.013.1384081
TCTAGAC33250.013.05151753
TTATACT52600.012.9076844
Produced by FastQC (version 0.11.5)