FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405375

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405375
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11566999
Sequences flagged as poor quality0
Sequence length79
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG20550.018.2874531
GTGCTAG38150.016.3542041
GTTCTAG46250.016.0933441
GTATTAG38350.015.1272361
GTATAGG23100.015.005121
CGAGACT45300.014.0949634
GTCTTAG37550.014.0891861
GTCCTAT30300.013.9682891
GTCCTAG32400.013.73860551
GGTCTAG27250.013.6571951
GCCCTAG30200.013.6520971
GCGAGAC46750.013.5014713
GTACTAA33350.013.2378471
GTGCAGA74250.013.2185451
CTAGACT37100.013.0798234
GAGCGAG51150.013.053691
GTATAAG36100.013.0379811
GTCCTAA33350.013.019041
GTGCTGG188450.012.6622091
GTTCTAA45800.012.586921
Produced by FastQC (version 0.11.5)