FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405376

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405376
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11528892
Sequences flagged as poor quality0
Sequence length79
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG21700.019.0113471
GTCCTAG35850.016.29391
CGAGACT46850.015.0362734
GCGAGAC45800.014.9028253
GTCTTAG36000.014.5019951
GTCCTAA30850.014.2010441
GTATTAG38850.014.1899271
GTGCTAG39700.014.1619941
GTTCTAG44600.013.9976611
GTACTAA32150.013.7403751
GTGTTAG42900.013.3609261
TATACCG4202.5083864E-913.0357595
GTATAGG24650.012.8853591
TGTCGCC17350.012.832396510
CTAGACT33850.012.723774
GTCCAGG81050.012.7025321
GTACTAT32650.012.6354131
GTATTAA56350.012.5042491
TCTATAC36250.012.3848163
GTACTGG42450.012.3845151
Produced by FastQC (version 0.11.5)