FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405377

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405377
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11528892
Sequences flagged as poor quality0
Sequence length79
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG20550.022.3656251
GTCCTAG34450.018.4239331
GTATTAG36750.017.5686441
GTTCTAG41300.016.5163441
GTGCTAG37750.016.3302211
GGTCTAG29800.015.7904951
GTCTTAG33500.014.9175691
GCGAGAC45500.014.0303283
GGTCTAA28350.013.6387671
GTACTAA31000.013.5318911
GCCCTAG32500.013.3562921
GGCTTAG35050.013.1130841
CGAGACT46600.013.0733464
GTGCAGG77400.013.0545271
GTACAGG43450.012.9286531
GTACTAT34450.012.8120451
TCTGTAC53250.012.8104123
GTGCTAA37550.012.7257591
GTCCTAT29400.012.6553781
GTCTTGG58850.012.6446271
Produced by FastQC (version 0.11.5)