FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405380

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405380
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10214755
Sequences flagged as poor quality0
Sequence length79
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG17600.018.4657631
GTATTAG33050.017.2362611
CGAGACT43100.016.4288064
GCGAGAC44000.016.341623
GTCCTAG30700.015.8198811
GCTCTAG28650.014.5301531
GTCTTAG30450.014.3907661
GTGCAGG70500.014.0886621
AGCGAGA51650.013.7094962
GAGCGAG50750.013.6712271
GTCCAGG73600.013.3960251
GTTCTAG37550.013.128461
GTCTAGC24800.013.1047361
GGTCTAA23650.012.9699431
GTCCAGA47950.012.9464371
TATACGG4001.774788E-812.7746685
GTACAGG38250.012.6972641
GTCCTAA28000.012.6503831
GCAAGAC75850.012.60745053
GTGCAGA67350.012.5246131
Produced by FastQC (version 0.11.5)