FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405381

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405381
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10214755
Sequences flagged as poor quality0
Sequence length79
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCTAG31300.018.4241451
GCGAGAC41200.015.5021533
GTGCTAG32700.014.9565551
GTATTAG31350.014.9020841
GTTCTAG38550.014.8644841
GTACTAG17800.014.7634231
CGAGACT40850.014.5625764
GTACAAG30300.014.0934661
GGTCTAG22850.014.0563011
GTCTTAG32800.013.6869231
GTACTAT28300.013.6708031
GTACAGG39250.013.5764981
GTACTAA28250.013.56581
GCGCTAG4656.002665E-1113.3435241
GTGCAGA63900.013.194281
GTCCTAT24900.013.1922161
GTATAGA35500.013.1600091
TCTGTCG16250.013.0261228
GCTCTAG30050.012.9961241
GTGTTAG41600.012.985031
Produced by FastQC (version 0.11.5)