FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405382

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405382
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15736675
Sequences flagged as poor quality0
Sequence length35-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG19250.017.0108491
GTATTAG30950.016.9284041
GTCCTAG38450.016.4950851
GTATTAA44850.015.6015121
TACGCGA2959.573341E-915.1898832
GTACTAA27300.015.1513621
GTTCTAA44850.015.1403841
CGATCGT4850.014.21628938
GTCTTAG33900.014.0317711
GTATAAG29700.013.9270111
GTGCTAG34900.013.728481
GTATATA54650.013.4976041
GTCTTAA40500.013.3621921
CTATACT35250.013.2988134
GCTCTAG49200.013.1712151
GTACATA43400.013.1047071
GGTCTAA28250.013.0556491
TCTAGAC35200.013.02393153
GTATAGG23850.012.5737261
GTATTAT45550.012.5618281
Produced by FastQC (version 0.11.5)