FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405383

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405383
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15736675
Sequences flagged as poor quality0
Sequence length35-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCTAG39750.017.3985561
GCGATCG7050.016.13626737
GTATTAG32000.015.8850181
GTTCTAG47350.015.7014171
GTATTAA46050.015.3186651
GTCTTAG34900.015.2586341
TAGTACG4350.015.0700244
CGATCGT7350.015.00948138
GTGCTAA39600.014.7574691
GTCCTAA33200.014.4776171
GTACTAG17100.014.1554051
CGAATCG7600.014.0589159
GTTCTAA45550.013.96851
GTACTAA28000.013.4613571
CTAAGCG10900.013.2945124
GTGCTAG41250.013.2450461
GCTCTAG47900.013.0666251
GTACAGG71200.013.0159281
GTGTTAG41250.012.9935581
GTATTAT43050.012.8518961
Produced by FastQC (version 0.11.5)