FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405384

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405384
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15608998
Sequences flagged as poor quality0
Sequence length35-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG30850.019.326681
GTCCTAG38950.016.45781
GTATTAA44000.015.8221321
GTATAGG25750.015.1240291
GTACTAG20150.014.8802591
GTTCTAG43000.014.8275581
GTGCTAG38600.014.4641881
GTACTAA28700.014.2899711
GTCTTAG36650.014.1053411
GTATATA52050.014.03723051
GTTCTAA45050.013.8468211
GCTCTAG42150.013.6547971
GTCCTAT32850.013.5338281
GTCTATA28850.013.2599991
GTATAAG31300.013.1029451
GTATATG45750.012.5049881
GTGCTAA35050.012.4876881
GTATTGG39000.012.4600791
GTGTTAA43100.012.3942641
CTATACT34550.012.3691534
Produced by FastQC (version 0.11.5)