FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405385

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405385
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15608998
Sequences flagged as poor quality0
Sequence length35-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG251710.1612595504208534No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG19850.018.8104531
GTTCTAG46700.016.2129941
GTATTAG29600.015.8848531
GTCCTAG41550.015.8095171
GTATTAA45350.015.1709161
GTACTAA27100.014.9263141
GTCTTAG34050.014.2150031
GTGCTAG38950.014.2019691
GTTCTAA46150.014.1587891
GGTCTAG31200.014.0729491
GGTCTAA30050.014.036261
GTCCTAA35900.013.675081
GTATAGG25450.013.5846431
GTATACG5950.013.364321
GTCTTAA38450.013.0379011
TCTATAC31750.012.9391623
GTATATA50150.012.6847791
GTATAAG29850.012.6246171
TCTAGAC33400.012.6100343
GCTCTAG45350.012.5788991
Produced by FastQC (version 0.11.5)