FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405386

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405386
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15797568
Sequences flagged as poor quality0
Sequence length35-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG19400.017.9468731
GTATTAG30300.016.7241421
GTCCTAG38150.015.3611431
GTCCTAA33650.014.956741
GTACTAA27550.014.7648681
CTATACT35600.014.3297834
GTGCTAG39050.014.2126151
GTATATA54300.014.1570961
GTATAGG24650.013.8448241
GTACAGG71900.013.71217251
GCTCTAG44400.013.664661
GTATTAA44250.013.5551751
GTTCTAG43550.013.4564311
TCTAGAC35650.013.2461283
GTCTTAA40550.013.0067781
GTATTAT45650.012.9884351
CTAGACT34900.012.9381984
GTACTAT29750.012.6301561
GTCTTAG38600.012.5921821
GTATAAA58900.012.5832361
Produced by FastQC (version 0.11.5)