FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405388

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405388
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15564379
Sequences flagged as poor quality0
Sequence length35-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAATCG5750.016.1854699
GTACTAG17950.016.1321241
GTATTAA44100.016.102951
GTATTAG29250.016.0283871
GTCCTAG37300.015.7114711
GTCTTAG35850.015.3853571
GTACTAA30050.015.3722371
GTTCTAG45500.015.3044151
GGTCTAG28850.015.0557191
GTCCTAA31450.014.6879411
GTATATA53950.013.99361
CGATCGT5050.013.65396138
GTATAGG25250.013.65261
CTATACT36350.013.5599964
GTGCTAG39650.013.3022461
TTGCGCG6000.013.21347612
GTTCTAA45150.013.1325021
GCGCTAA6050.013.1053691
TATACTG44350.013.0570055
GTATAAG31300.012.99614051
Produced by FastQC (version 0.11.5)