FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405390

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405390
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16220529
Sequences flagged as poor quality0
Sequence length35-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG19850.017.3655321
GTCCTAG38850.016.0596521
GTATAGG26250.015.3640311
GTTCTAG43650.014.8464361
GCTCTAG43850.014.62151
GTACTAA31850.014.6107641
GTATTAA47500.014.368791
GTGCTAG39600.014.2756961
GTATTAG31000.014.2330131
GTACAGG72100.014.1993921
GTCTTAG36250.014.0735051
GGTCTAG29200.013.6937931
GTATATA57000.013.6068091
GTCTATA31350.012.9745731
CTATACT36250.012.932414
GTATAAG31450.012.8237131
TCTAGAC35000.012.5078953
GTGTTAG40300.012.4881011
GTCCTAA34600.012.4532451
TCAGTAC44050.012.285773
Produced by FastQC (version 0.11.5)