FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405394

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405394
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15931237
Sequences flagged as poor quality0
Sequence length35-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCTAG37400.016.0380331
GTATTAG32200.015.8445281
GTCTTAG35600.015.4932831
GTACTAG18900.015.3211341
GTTCTAG46650.014.8531261
GTATTAA44150.014.679141
GGTCTAG31650.014.2682611
GTGCTAG39800.013.6850841
GTACTAA30350.013.6300041
GGTCTAA30100.013.399631
GTGTAGG58150.013.1606311
GATCTAG30000.013.099571
CTATACT36400.012.974564
GTACAGG71450.012.8819761
GTTCTAA45250.012.7224671
TCTAGAC35850.012.6928223
GTCTTAA41300.012.6872351
GCCCTAG39100.012.5194441
CTAGACT33900.012.507744
GCTCTAG45650.012.4599591
Produced by FastQC (version 0.11.5)