FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405398

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405398
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13058819
Sequences flagged as poor quality0
Sequence length35-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG25100.018.2365671
GTCCTAG31200.016.987571
GTGCTAG32400.015.1899441
GTACTAG15650.014.9540821
GTACTAA21950.014.5818831
GTCCTAA26900.014.4574381
GTATTAA37900.014.1661091
GTCTTAG28700.014.030371
CGATCGT5400.014.02567738
GGTCTAG25850.013.9795731
GTATAAA48350.013.4533521
GTGTTAG30050.013.2855231
GTCTATA25000.013.0782241
GCCCTAG31950.012.8186211
GTATTGG30450.012.6588971
GCGATCG5750.012.57215637
GTACATA32850.012.4674241
CTAGTAC14950.012.4313343
GCTCTAG35850.012.3841371
GTTCTAG35600.012.3744291
Produced by FastQC (version 0.11.5)