FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405402

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405402
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12818001
Sequences flagged as poor quality0
Sequence length35-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG22650.017.3210281
GTCCTAG28400.015.874131
GTCTTAG29900.015.4230621
GTGCTAG29500.015.3988711
GTACTAG16050.015.0092851
GGTCTAA20800.014.8907351
GTATTAA38100.014.4521331
TATACTG34450.013.8855875
GTGTTAG31050.013.6326561
GTATATA39150.013.6250121
CTATACT28050.013.6184334
CGAATCG6350.013.548039
TACGCGA2801.3602566E-613.5198422
GTTCTAG35450.013.493841
GTGCTAA31050.013.3001521
GTATTAT36900.012.8703311
GTCTATA23000.012.8678971
GTACTAA21150.012.8544551
GGTCTAG25050.012.7765091
GCTCTAG36050.012.6964811
Produced by FastQC (version 0.11.5)