FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405403

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405403
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12818001
Sequences flagged as poor quality0
Sequence length35-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG14700.019.4539471
GTCCTAG31450.016.871241
GTACTAA23400.016.4910831
GTGCTAG32550.015.2425811
TCTAGAC27850.014.9524413
GTCTTAG27950.014.9159371
GTTCTAA35450.014.6761
GCTCTAG39450.014.6726741
GTTCTAG36600.014.3090071
GTATTAG22200.014.2784761
GCGATCG5800.014.24199337
CTAGACT26600.014.1007064
GTCCTAA26200.014.0711461
GTATATA39700.013.79919051
CGATCGT5850.013.53313638
GTACTAT23700.013.5201521
CTAGGGT25600.013.4417574
GCCCTAG31500.013.2349351
GTATAGG20850.013.2199841
GTATTAA36100.013.1710041
Produced by FastQC (version 0.11.5)