FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405404

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405404
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12819986
Sequences flagged as poor quality0
Sequence length35-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG13900.020.0512371
GTATTAG22850.017.4679811
GTCCTAG29600.015.80951
GTTCTAA35000.015.3365441
GTATTAA34500.015.2596041
GTATAGG20600.014.6989531
GTATATA40250.014.2764921
GTGCTAG31850.014.2605231
GCTCTAG39400.014.0604931
GTTCTAG32950.013.8888781
GTATTGG29400.013.5763051
GTACTAA24450.013.0880531
GTCCTAA25350.013.0305951
GTCTTAG26750.012.8631441
GTACAGG57450.012.6974571
GGTCTAA24050.012.590371
GGTCTAG24500.012.3591191
GTATTAT36550.012.3326051
CTATACT27650.012.3202494
GTCTATA22450.012.2615281
Produced by FastQC (version 0.11.5)