FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405405

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405405
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12819986
Sequences flagged as poor quality0
Sequence length35-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG13900.020.8149171
GTACTAA22500.017.4514921
GCGATCG4800.015.77488737
GTGCTAG30450.015.4968531
GTCCTAG33000.015.2387441
CGATCGT4750.015.21741938
GTCTTAG27100.014.8705431
CGAATCG4900.014.7456529
GTATTAA37150.014.0927271
GTATATG34600.014.036321
GTATTAG24300.014.0326331
GTCCTAA25550.013.8853411
GGTCTAA20850.013.381021
GTTCTAG35950.013.3176061
GCTCTAG38200.013.164361
GCCCTAG33600.013.0189121
TACGCGA2701.3836154E-512.7503672
GTTCTAA35400.012.6488291
CTAAGCG9600.012.5456114
GTATAGG19850.012.4934461
Produced by FastQC (version 0.11.5)