FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405406

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405406
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7072313
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGTCTA53650.022.263538145
GTCCTAG27000.020.3234371
GTACTAA26700.016.6697881
GTACTAG15950.016.054881
GGGGGGG347850.016.024284145
GTATTAA47000.015.8263371
GTACATA38950.015.8100181
GTATTAG29700.015.8071181
GCCCTAG26750.015.7269221
GTATAGG21600.015.5248491
GTATATG41950.015.2607461
ATAGTAC25450.015.0929563
GTGTAGG38600.015.0056481
GTATATA55450.014.9539461
GTGCTAG28200.014.9182681
TAGTCGG9550.014.6814589
GTTCTAG33200.014.3243511
GTATAAG30400.014.2397781
CTACACT31650.014.0627634
GTACAGG49050.014.0461671
Produced by FastQC (version 0.11.5)