FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405407

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405407
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7072313
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG28100.021.7221851
GTCCTAG30150.019.8403171
CTGTCTA50900.019.742369145
GTCCTAA27200.019.0747951
GTACTAA25600.018.5979231
CGACGAA9902.426701E-618.270662145
GTCTTAG29150.017.3799841
GTACTAG15400.017.0434511
GTTCTAG35500.016.5064131
GTTCTAA41000.015.9297781
GTATAGG20000.015.8702291
GGTCTAG20750.015.5907721
GTATATA55600.015.1500511
TTATACT48500.014.7213114
GTGCTAG28750.014.6494411
GTCCTAT28300.014.4510111
CCCTTAG27600.014.3752071
GTATTAA45900.014.3621981
GTATAAG28650.014.2744691
TACTAGG19350.014.1916832
Produced by FastQC (version 0.11.5)