FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405408

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405408
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7110364
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGTCTA51750.036.047256145
GTACTAA26350.019.2307191
GTCCTAG26550.019.0858521
GTACTAG16000.017.5523741
CTATACT29400.016.8210854
GTACTAT25250.015.7162891
GTATTAA46000.015.3956561
CTGTACT58850.015.1468644
GTATAGG23050.015.0973921
GTATTAG29450.014.926061
GTCTTAC34750.014.7578291
GTACAGG51400.014.6096561
TATACTG42450.014.5264675
GGGGGGG326900.014.448298145
GGTCTAG22100.014.3651141
TCTATAC30150.014.1746323
GTATATG40750.014.0830981
GTACATA40450.013.7347541
GTCCTAT27600.013.71451951
CCTATAC20500.013.699513
Produced by FastQC (version 0.11.5)