FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405409

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405409
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7110364
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGTCTA49850.022.126013145
GTGCTAG29850.018.8347591
GTCCTAG27350.018.7688871
GTACTAG15900.018.4484861
GTTCTAG35450.018.2728441
GTATTAA48100.018.1680181
GTACTAA26150.017.9943281
GTCCTAA29000.017.4902761
GGTCTAG22350.016.6789571
TTACGCG17050.016.466805145
GTACAGG49500.016.4196171
GCTCTAG30750.016.2961621
GTCTTAG28950.015.1984911
GTCTTAA35650.015.08480551
TCTAGAC26400.014.8122963
CTAGCGT4958.0377504E-814.8122964
TACGCGT16351.8189894E-1214.718688145
GTCCTAT27900.014.6753071
GTTCTAA39400.014.5796871
GTACATA37450.014.3597671
Produced by FastQC (version 0.11.5)