FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405412

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405412
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7037665
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGTCTA55300.044.457935145
GTATTAG28750.021.84951
GTACTAA26700.018.9587171
GTACTAG15700.018.6459141
GTGCTAG30400.016.4506241
GTTCTAG36500.016.2076851
GTCTTAG28900.016.0382821
GTGTTAG32500.015.95062351
TATACTG36650.015.8103375
GGGGGGG320150.015.792084145
GTTCTAA37200.015.7387561
GCTATAC20100.014.5644293
GTGTAGG38750.014.4796551
GTATTAA43100.014.433281
GTATACG6406.311893E-1014.2939861
CTATACT28450.013.93520454
GTATATA54450.013.8888381
TGTACTG47950.013.8653035
ACGGACG10050.001129392713.809715145
GTCTAGG26500.013.8085311
Produced by FastQC (version 0.11.5)