FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405413

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405413
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7037665
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGTCTA54950.033.440605145
CCTGTCG21800.023.821598145
GTACTAG14900.019.2611581
GTATTAG27250.018.150531
GTATTAA46050.017.9009021
GTTCTAG37150.017.7515281
GTCCTAA26000.017.1443211
GTTCTAA40500.016.4339621
GTCCTAG27800.016.2539041
GTATATG41500.015.8908251
GTACTAA25750.015.8879681
GCCCTAG25300.015.2051531
TACGCGG8050.00394889914.887074145
GTGCATA31800.014.7854451
CCTAGAC27300.014.7621283
CTAGACT25300.014.7223454
GTATAGA26600.014.6916021
GTGCTAG29250.014.613121
GTATATA50750.014.5586161
GGTCTAA23150.014.5071631
Produced by FastQC (version 0.11.5)