FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405415

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405415
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12030703
Sequences flagged as poor quality0
Sequence length35-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG12400.019.5029341
GTATTAG19950.017.836841
GCGATCG4700.017.59154137
GTCTTAG26050.016.9756281
TACGCGA2502.0307198E-816.5668352
GTCCTAG27500.016.4574361
GTTCTAG33600.016.0401691
GTACTAA20400.015.9192011
GTTCTAA30950.015.8508041
CGATCGT5450.015.80417938
GTATTAA32250.015.2118571
GTATAGG18750.015.1090151
GTACTAT18550.014.3407021
GTATATA31850.014.2097171
GGTCTAA19750.013.9941481
GCTCTAG34750.013.918641
GTCTTAA30800.013.90895751
GTGCTAG28000.013.81922151
GTCCTAA26850.013.7677531
TATACTG32850.013.7398075
Produced by FastQC (version 0.11.5)