FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405416

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405416
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12195138
Sequences flagged as poor quality0
Sequence length35-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCTAG29350.017.3699231
GTACTAG13300.017.093721
GTATTAA31600.016.569171
GTATTAG19450.016.1163251
GCTCTAG33650.015.5597551
GTATATA35600.015.2880251
GTATTAT30950.015.1363961
GTGCTAG29600.015.0121271
GTCTTAG25600.014.2629771
GTACTAA21500.014.2592211
GTCCTAA24650.014.25369551
CTAGTAC12600.014.2165133
GTGCTAA27000.013.6509931
CTAGACT27250.013.400174
GTCCTAT24450.013.2432091
TCTAGAC25100.013.1752173
GTTCTAA31450.013.1433121
GTATATG33000.012.9435091
TCTATAC24550.012.909123
TATACTG33250.012.8470315
Produced by FastQC (version 0.11.5)