FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405417

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405417
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12195138
Sequences flagged as poor quality0
Sequence length35-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG12100.017.9882091
GTATTAG21950.017.471141
GTCCTAG31850.016.813381
GTATTAA30000.016.353091
GTATATA35950.016.2412931
GCGCTAG4500.015.3550161
GTGCTAG28750.015.2615491
GTCTTAG28400.015.2063321
GGTCTAG22650.014.948261
GTTCTAG36250.014.2960491
GTACTAA20950.014.1823181
CTATACT26400.014.1005424
GTCCTAA24900.014.0137641
GGTCTAA22050.013.9448611
CTAGACT26700.013.9421084
GTACATA30250.013.9337241
GTATAGG17950.013.6655361
GTTCTAA31900.013.6462291
CTAGACG6850.013.5859244
TCTATAC24650.013.573663
Produced by FastQC (version 0.11.5)