FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405418

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405418
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12151794
Sequences flagged as poor quality0
Sequence length35-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG13150.021.221191
GTATTAG20400.018.0702461
GTCCTAG29800.017.8038881
GTACTAA20100.016.6259371
GTATTAA30600.015.9873821
GTTCTAG34150.015.435161
GTCTTAG26200.015.3849121
GTGCTAG28150.015.0534891
GTATATA34600.014.7365581
GTATAGG20200.014.3264421
GTCCTAA26800.014.1406161
GTTCTAA30200.013.9175661
TCTATAC23200.013.5145983
GTATAAG22150.013.3762741
GTATATG31900.013.1758781
GGTCTAG21700.013.0186081
GGATTAG25350.012.9108881
GCTCTAG35700.012.6419341
GTCCTAT27300.012.4934681
GTACTAT19600.012.4799461
Produced by FastQC (version 0.11.5)