FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405419

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405419
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12151794
Sequences flagged as poor quality0
Sequence length35-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCTAG29450.016.3927231
GTACTAA22250.016.2729991
GTATTAG21200.016.265691
GTACTAG12200.015.5457331
GGTCTAA21600.015.4855391
GTATTAA31750.014.9880021
GTTCTAG32550.014.83151151
GTGCTAA26500.014.18368151
GTCTTAG26900.014.1009631
GTCCTAA25800.013.900231
GTCTTAA29250.013.5575241
GTACTAT21600.013.4101581
GTGTTAG27550.013.3927741
TTATACT38000.013.146234
CTAGACT26600.012.951954
GTATATA35450.012.9373031
TATACTG34950.012.8146745
GTCTAGA27000.012.7715791
GTGCTAG27300.012.7575441
CTATACT27250.012.6430044
Produced by FastQC (version 0.11.5)