FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405421

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405421
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12020659
Sequences flagged as poor quality0
Sequence length35-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG19350.018.5304851
GTACTAG13850.018.17221
GTACTAA20400.017.069691
TAGTGCG6200.016.4087457
TCTAGAC25450.015.974883
GTATAGG16600.015.784831
GTATTAA31900.015.6715411
GCGTTAA3151.373337E-915.3232861
GTCCTAG31100.015.298651
GTTCTAA30950.015.0386041
GTCCTAA24650.014.8259781
GTCTTAG24800.014.319241
GGTCTAG25250.013.9275021
TAGTACT21550.013.7481414
GTTCTAG33400.013.6257951
GCGATCG6350.013.56468437
GTATATA34950.013.3174481
CTAGACT25950.013.1428834
GTGCTAA27750.013.04551
GTATTAT31500.013.0247941
Produced by FastQC (version 0.11.5)