FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405422

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405422
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15425726
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG28600.018.973891
GTACTAG18500.016.464111
GTCCTAG40350.016.3119181
GTATTAA43700.015.7023761
GTACTAA27800.014.8603131
GTGCTAG37850.014.7069271
GTTCTAG45400.014.5746011
GTATATA53000.014.466351
TCTAGAC36400.014.4272463
GTCTTAG32400.014.26328851
GTATAGG25650.013.785571
GCTCTAG43800.013.428451
TGTACTG62900.013.1914965
GTTCTAA45050.013.1335651
GTATAAG28900.012.9621391
GCCCTAG34100.012.9362061
GGTCTAG29550.012.9139691
GTACAGG66200.012.9039471
GTATAAA61200.012.8140761
GTACAAG53400.012.784511
Produced by FastQC (version 0.11.5)