FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405423

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405423
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15425726
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG19250.018.0349241
GTATTAA43250.017.1893561
GTCTTAG33550.016.8284171
GTCCTAG38350.016.8252811
GTATTAG32300.016.5025461
GTTCTAG42700.015.7682391
GTCCTAA32450.015.4536551
GTTCTAA46100.015.2138441
GTACTAA28700.014.7847271
GCCCTAG34350.014.7009571
GTGCTAG36650.014.6395361
GTATATA50900.014.6058871
GTATAGG24850.013.8295971
GGTCTAG28700.013.8072251
CTATACT37950.013.65123754
GTGCTAA34900.013.4644681
GCGCTAG6350.013.2540121
GTACAGG65100.013.2514681
TCTGACG107050.013.17524970
GTATTAT45300.012.773081
Produced by FastQC (version 0.11.5)