FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405424

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405424
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15374259
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG19000.017.3173311
GTCCTAG38250.017.2956561
GTATTAG29150.017.051261
GTATTAA47550.015.6060161
GTATAGG24600.015.08264351
GTCTTAG32150.015.0246661
GTATATA53750.014.7827111
GTACTAA27950.014.7777021
GGTCTAA27850.014.4537111
GTGCTAG36300.014.1748151
GTATAAG31500.014.0012461
CTATACT34850.013.6600194
GTACAGG66700.013.4344791
ATTAGAC30750.013.4322653
GTATTAT42550.013.2444231
GCTCTAA39100.013.0702171
GTTCTAG46150.012.8938891
TCTATAC31850.012.8584553
GTATAGA32700.012.7381361
GTACATA44150.012.6851611
Produced by FastQC (version 0.11.5)