FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405425

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405425
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15374259
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCTAG36650.018.6547131
GTACTAG18700.018.3743691
GTACTAA29550.018.0349121
GTATTAG28700.016.614411
GTGCTAG35100.015.283131
GTCCTAT35350.015.2742261
GTCCTAA33300.015.0563521
GGTCTAG30950.014.6135921
GTCTTAG34850.014.4873061
GGTCTAA29000.014.3872211
GTATTAA46000.014.3293981
GCCCTAG37250.014.30689051
TCTATAC35800.014.1797373
GTTCTAA44950.013.7281151
GTTCTAG46250.013.7212841
GCTCTAG44100.013.5951961
GTACATA44200.013.4851131
GTATATA51350.013.45097351
GTATAAA59150.013.33692551
TCTAGAC35950.013.2441233
Produced by FastQC (version 0.11.5)