FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405427

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405427
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15160702
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG31300.019.468991
GTCCTAG36250.018.2596451
GTACTAG17850.016.6770521
GTGCTAG36750.016.1052671
GTCTTAG35350.016.0495971
GTATTAA47000.015.5735271
GTCTTAA40300.014.6865641
GGTCTAA28500.014.623141
GTTCTAA46100.014.5101191
GTATATA52200.014.4246911
GTCCTAT32700.014.0301441
GTTCTAG41750.013.8409541
GTCCTAA30550.013.4126071
GTACTAA26950.013.3849581
GTATAGG24600.013.3823161
GCTCTAG42950.013.2096221
GTACATA43750.013.0481241
CTAGACT32900.012.9778144
GTATACG5700.012.9027721
GTACTAT29550.012.7998491
Produced by FastQC (version 0.11.5)