FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405428

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405428
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15172352
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG16750.019.0156921
GTATTAG30500.017.4433251
GTACTAA28850.016.8637641
GTCCTAG37350.016.3058781
GTATTAA44850.014.3595531
GTTCTAG42300.014.3149981
GTATATA54850.014.2941041
GCTCTAG40900.014.2059531
GTCCTAA30850.014.1820951
CTATACT37200.014.0197074
GTATTAT45550.013.9851951
GCGCTAG6150.013.6590871
GTACTAT27750.013.622171
GTGCTAG35550.013.4885881
CTAGACC30250.013.42233854
GTCTTAG36550.013.4068321
TCTATAC34100.013.3436913
CTAGACG8250.013.15234854
GTATAAG29850.013.01561
TCTAGAC34350.012.9408853
Produced by FastQC (version 0.11.5)