FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405429

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405429
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15172352
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCTAG37900.016.724061
GTCTTAG34850.016.2785071
GTATTAG31350.015.9735281
GTACTAG18650.015.9603511
GTACTAA30150.015.2155031
GTATTAA44250.015.1154981
GTGCTAG37050.014.6502771
GTTCTAA44850.014.6009641
GTTCTAG43700.014.4242551
GCTCTAG43950.013.9438111
GTACATA43750.013.367211
TCTATAC31400.013.2646673
GTCTTAA41050.013.2227221
GTGTTAG33900.013.222471
CTATACT37200.013.1722454
GGTCTAA28800.013.1320831
GGTCTAG29400.013.1023041
GTATATA54350.013.01531
GCGATCG5650.013.00866337
GTATTAT44300.012.8060041
Produced by FastQC (version 0.11.5)