FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405448

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405448
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1953912
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGAATTTCGACGATCGTTGCATTAACTCGCGAAGGGTCAAATCTCGTAT30433315.575573516105127No Hit
TATTTGCCCTCCATTAGCGAATTTCGACGATCGTTGCATTAACTCGCGAA645293.3025540556585966No Hit
GCCGCCCATGCGCGGGCATGATGAACTCCGCTGCTGTGGTATCGCCCAGT400362.049017560668034No Hit
TTACCTGCTCTCCCTTTCCGCCGCTGCTCGTCTTCTTGCTCTGTCAGGTA296341.5166496751133112No Hit
CCAGGCTCCTGGAGCACCCCAAAAGAATTGCAAGGCAGTCCCAAGTTTTG202871.0382760329021983No Hit
CACCGAGCAGCAAACAGAAGGGACTTCCGTTACCTTCTCTCCTCTTAAGT193320.9893997273162763No Hit
CACAGCAGGCCAGTGTGCAGGGTGGCAAGTGGCTCCTGACCTGGAGTCTT176100.901268839128886No Hit
TTTCTGACTGGCTGGGGGATGGGGCCATGCCATTGACATCTGGGCTTTGT158490.8111419552159974No Hit
AAGAGGCATCGGGTCCGCAGGCCTGGGGCGCTGAAGGGTTGCAGCCACAT158040.8088388832250378No Hit
TTTGTGACCTACCAAGATGCCCTGGGGTTGGGCATGGTGCATCCCATGTT156430.8005990034351599No Hit
ACGGTGAGTCCACGGATACAGCCTCTGACTTTGAAGGTCACCTCACGGAG154550.790977280450706No Hit
TAAGGAAAAGCTGGCCAAGAACGGCATCTGCCAGAGAATCTTCGGGGAGA153770.7869852889997093No Hit
GAAAGCGCTCCCCATTGGTGCAGACTGGAGAATACAGCGGCTGGGCCAAG149290.7640569278452664No Hit
TGGCCCAGGGCGGTCCCACAGCCGCGCGCCTCACGCGCAGTTGCCCATGG140230.7176884117606116No Hit
AAGCTACCCTGCAGCAACTGCATCACAAGTGGGTTAGTGGCCTCCACTGA137780.7051494642542755No Hit
CCTGGGACTTCTTCCTCCGTGCCTTGAGGTCCTTGGCCTCCTTGCTTCCA128700.658678589414467No Hit
AGAGCTGAGGAAGCCGGCGCCAGTTCGCGGGGCTCCGGGCCGCCACTCAG128290.6565802349338149No Hit
GTAGGGCGGCGGCAGGTAGGTGTGGTAGCGCGTGGCCGAGCCCATGGCCG122420.6265379402961853No Hit
TCCCTGTGGAGGGGACCCTGCCCCTGGTTACCACTAACTTCAGTCCTCTG122300.6259237877652627No Hit
AACCTGGGGCTCAACAGATGGTATGTGTTCTCTGGATTCTGGTTTGGGCT120870.6186051367717686No Hit
ATTTTTGGCTGGGGATCGTCAGGCTTGTCTTCTGACTTGTGCCCAAGTTT116850.5980310269858622No Hit
GCTCTTTTGCTGCACATTATCACAGCTTGCAGGTGGATTCTCTTGCTTAG112070.5735672845041128No Hit
CCGAGGTTTCTCAGAGCCTCTCTGGTTCTTTCAATCGGGGATGTCTGCAG111330.5697800105634235No Hit
GCGAATTTCGACGATCGTTGCATTAACTCGCGAAGGGTCAAACCTCGTAT110450.565276225336658No Hit
CCTCATCGCTTCCTGTCACCTGCAGGGTGCCGCTGAGCACCGCCTGGAGA110000.5629731533456982No Hit
ATTGTCTGCAGGAACGGTGGGTCCTTGCTCCTCATCATCACTTTCCCAGG109760.5617448482838531No Hit
TGAATTTCACTGTGGGCGGGTCCACCAGTTTGAATGGTCAATGCGAGTGG109730.5615913101511225No Hit
GCTGTAACTTTTGGTAAAACCCAAGAGGATCCCAAACCATTTTGTGTGGG109080.5582646506086251No Hit
GTCCTCAGTGGAGGCCACTAACCCACTTGTGATGCAGTTGCTGCAGGGTA107350.5494106182878247No Hit
GGTAACTAAGCAAGAGAATCCACCTGCAAGCTGTGATAATGTGCAGCAAA107020.5477216988277875No Hit
CCCTTCGCGAATTTCGACGATCGTTGCATTAACTCGCGAAGGGTCAAATC105080.5377928995778725No Hit
TGCGCCCCGGACTTTTCGAGGGCCTTTCCTACCTGGTCTTCTAGGAAGCG104220.5333914731062607No Hit
CTCTTGGCGGGCCTCACCCCCAGCCCCAAGTCCTATGAGAACCTCTGGTT100240.5130220808306617No Hit
GGAAGACGGGCTGCGCCCCACAGCATGGACATACATGCTTCTGTGTGACG99310.5082623987160118No Hit
GGCCACGGCTGGTGAAGAAGCCGCTCACCTCGTACTCTGGCTCGTCATCG99150.5074435286747817No Hit
CGGGACAGCCCCGGGACTCTGCCAGGTGGATGTTGTGCGTAGCCGGAGCC98550.5043727660201688No Hit
ATAGGGGCTGGCAGAGGACTGAAGTTAGTGGTAACCAGGGGCAGGGTCCC97060.4967470387612134No Hit
GACCTCAGACCTGACCCTCTACCTCTCCACAGTGCCGGAAGCTGCCCAGT91870.4701849417988118No Hit
AAGTGGCGAAGCCGTAGGTGCGCACGGGCGGTGGACGGAGATCTGGGGTC91540.4684960223387747No Hit
GACAGCCACTCGCATTGACCATTCAAACTGGTGGACCCGCCCACAGTGAA91260.467062999766622No Hit
CAGCCAGATGCCAGCACAACCCGGGTACCTTTCCATTTCAGTGCACCATA90480.46307100831562525No Hit
TGTCGCTACCTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATACT88150.4511462133402118No Hit
GAAGCGAAAATTCCATGGGACTGACTTTCTGCTCTTGTCTTTCAGACTTC87390.44725658064436885No Hit
GGTGTCTGCAGCAGGCTGGGTGGGAAGGTGGACTCGGGGTGACGGCCGAG85410.43712306388414623No Hit
GTCCTCCCACCACCCTCTCCGGGCCAGTACCTTGAAAGCGATGGGCAGGG85260.43635537322049306No Hit
CCCCCGGCAGGCGGGCGAAGCAGACAGGGCTCACCTTTAGAGACTCGTCG83380.42673365023603926No Hit
TGGGTTCTCATCACCACGGGCCCAGTGCTCACCGTGCGCGGGGCTGTAGG81240.4157812634345866No Hit
GACCATCCTCTGTACATCCCATCCACAGGAAGCATCTGTAGCTCCTTTGC80410.4115333750957054No Hit
AGTGGTCCTTCCAGGCACCGCTGCGGCCCACCTCATTTTTCACCTGTCTC78720.40288406028521245No Hit
TTCCCTTCCCTATGCAGCCCGTCCTCGGAGAGCCTGGACTGTTTGAAATC76210.3900380365134151No Hit
TTGAGGAGCATTGGAATTCCCAGGTCTGCACTTAACTAGGGAAAGTGGTC74380.3806722104168458No Hit
TCTGTGCTGGAGCCCCTCCTGCTGCTCGGAACCACGTCCAATATAAGTTC70380.36020045938609313No Hit
GCAGGAGAGAATTTAGCGATAGTTTGCAATTCTGATTAATCGCGTAGAAA70270.35963748623274744No Hit
TGAGAGGACAGTGGCCCCAAATTCAGCTGGCATATCTCTAGCGAGTCTAT68980.3530353465253297No Hit
AAACAGCATCTGCCGTCATCTGATGGACATGATTAGGAAGTCCCTCGACA66620.3409570134171856No Hit
TCTCCTCTGCTCACTGGGTCTCCTTCCAGGTGCTTTTGCGTGGAGTGTGT66050.3380397888953034No Hit
GGGGTCCCAGAAAGCTGGGTGCCCTCATTTACCTTCTGGTGGCTCCAGGC65260.33399661806672976No Hit
GGACCTGCTGGAGAGCCAAGTCCCTGACTCTCAGGGTATGCTGGTGGGCC64910.3322053398515389No Hit
GACATAAGTTTTTCAGTTTGGGAATCTGCTCTTTGTTGAAAATGAAATCT64850.3318982635860776No Hit
AAAGCCAGCTACAGATGGTACAGGTGACCGGCTCCTCAGATAATGAGTAC63880.32693386396112006No Hit
GCCCCCTCCCAGCCACCTGTGCCCGCTCCTACCAGTCTTCGCTTGGGCTT63530.3251425857459292No Hit
ACGTCCCCGCCGCTTGTTGTCTTGTAGGTCTCGGATCCGCAGAGAACTGT63280.32386310130650714No Hit
AGGTCAGCGGGCTACCACTGGGCCTCACCTCTATGGTGGGATCATATTCA62740.32109941491735555No Hit
TTGGCGTGAGCTCAGCAGCCAGCGGCCACCTGGGCCGGAGCTTCCTGAGT60700.31065882189167165No Hit
GGACTAGGTTTGTGGTTAACCAGAAGCAGATCTGTTTTCATGGGGCTCTG60630.3103005662486335No Hit
CAGCACCTCTTGGGCCTGCACCCCTCACCTGTAGCGATTCCATCAAAGAG59750.30579678102186797No Hit
GCCAGACCTGGAGGGGCGGGGCGGTGCCGGCAAGATGGCTGCGCCCGAGA59740.30574560164429104No Hit
CCAGTCCCATTTGGACATTATGAGTCTCGAACTCGCTTGATTTTGAATAC59300.3034937090309082No Hit
ACTCAGGAAGCTCCGGCCCAGGTGGCCGCTGGCTGCTGAGCTCACGCCAA59250.3032378121430238No Hit
GGATCCCGGCGGGCTTGTCGCGAACAGGAGGCCCGCGCGCCCGGAGGCGA58880.30134417517267925No Hit
AGCTGGCCAAACCAAGGTTGCTGGCTATACCTCGAGAAATCGCGAGATGT54730.2801047334782733No Hit
GCGAATTTCGACGATCGTTGCATTAACTCGCGAAGGGTCAAAACTCGTAT53880.2757544863842384No Hit
AGAGGGTCCCCAGCAGAGCCCGCTGCTCACCTTTCGGTCCTCCTCCCGCT53310.27283726186235613No Hit
CAGCCCCAGGGAGCGCCCACAGGGACACACCTCATAGCCAAGCCTGGCTG53260.2725813649744717No Hit
GGAGGGTGTACCAGCCCCAAGTGGATGCACTTACTTCGAGGTTCTCGGGG49720.2544638653122556No Hit
ACCACAAGCTTCATTCACCAAATCTGCCTGGTGCCCTGCCCTACCATACT49520.253440277760718No Hit
CAACGGCACCTCTCCTGGGTGGGTGTGCTCCTACCTTGCAGTTTTGGCAC49240.2520072551885653No Hit
CGAATTTCGACGATCGTTGCATTAACTCGCGAAGGGTCAAATCTCGTATG49130.25144428203521957No Hit
CAGATCTACAGCGAACACAACTACTGCCGCCTTATCCTCTAAATGGGGAG48990.25072777074914326No Hit
GGGCTGAAGGCTGTTCCCTGTTTCCTTCAGCTCTACGTCTCCTCCGAGAG48190.2466334205429927No Hit
CTGGCACCTGGACGGCGGCGCCAGGCTCACCTCTATAGTGGGGTCGTATT47930.24530275672599383No Hit
GGGTATGCTCCCCATTTAGAGGATAAGGCGGCAGTAGTTGTGTTCGCTGT47650.24386973415384114No Hit
CAGTTCCACAGAGGCAGGTGAGCAACAGCAGGAGGCCGAACAGGCCCAGG45580.2332756029954266No Hit
CTCTGGGAATGTGGCTGCAACCCTTCAGCGCCCCAGGCCTGCGGACCCGA45480.2327638092196578No Hit
AAAGCCAGCAACCCAGAACCGAGTTTCAAAGCAAACGAGAACGGCCTTCC44250.22646874577770135No Hit
CAAGACCCTCTGTCTAAGCTCAGTCTACCACCCATCCATACACTTTACCA43300.2216067049078976No Hit
TTTACCATCCAGGCCTATGCTGACCGTACACCCCTCCCAGCCGCCATCCT43150.22083901424424437No Hit
CCTAATCCATCTACACATGTATGCAGCCCTTCTCCGATGCTTTCTGAAAG42780.21894537727389973No Hit
TCCAGTTGCTTTATCTGTTCACTTGTGCCCTGACTTTCAACTCTGTCTCC42400.21700056092597825No Hit
ACATCCACCACTTTAGAAGACAAGTCTAGTGGCTTATCTGTGACGTCTTT41560.21270149320952017No Hit
CCTGGTCGCAGTGCCCGTCGCTGAAGTGGTCCTTGCAGTACTGGTCGTAC41120.21044960059613738No Hit
CTCCTACACCGGTGCCTGCACCCACCCCAGCCCCCATCTTTACTCCAGCC40560.207583555451832No Hit
TCAATAACTCCCATCCTTGCCTTTGCAGGCCCTGCAACTGGTTCCTCTTT40530.20743001731910138No Hit
CACACTGTACATGGTGGGTCCAGCTTGCTCACCAGTAGTTGTCTGAGGCC40490.20722529980879384No Hit
ACCCTTCGCGAATTTCGACGATCGTTGCATTAACTCGCGAAGGGTCAAAT39570.2025167970717207No Hit
GTTGTCTCTGATGATTACCTGCAGAGCAAGGAATGTGACTTCCAGACCAA38330.1961705542521874No Hit
AATTGAAGGCCCCTCGTTTTCACCAAGCGGAATCCCATCTAAAACGTAAT38270.19586347798672613No Hit
GTGAGACCCCCGCTCTGCCCCTTCTAGGATGCGGCCAAGCCCCTGGAGGT36700.18782831570715572No Hit
GCACGGTTTCTCGTGTGCTTTAATTACAGAAAGCACTCCAAAGACCTCCG35330.1808167409791229No Hit
GCGAATTTCGACGATCGTTGCATTAACTCGCGAAGGGTCAATCTCGTATG35130.17979315342758528No Hit
GTTGGAGTTGTGAATTCGCTGCGTTTCCATGAAATCCTGCGGAGTGTCGC34990.17907664214150892No Hit
TCCTCTCTTTCTCAGATAACCTGAGGACCATGGATGCTGATGAGGGTCAA34120.17462403629232023No Hit
GGAGTGCCAGAGTTCCCAGGCAACAAACTTACCCTCAATGTTCCGGCACT33730.17262804056682185No Hit
TATTTGCCCTCCATTCGCGAATTTCGACGATCGTTGCATTAACTCGCGAA33550.17170681177043795No Hit
ATCCTGGATACCGCCGGCCAGGAGGAGTACAGCGCCATGCGGGACCAGTA33340.17063204484132347No Hit
GGTGAGATCCATTGACCTCAATTTTGTTTCAGGACCTGCTTCGCTGCCGT32950.16863604911582508No Hit
CCGCCTCCCGCGGTCCCCTCAGCCCCGTTTACCTGCGGCTCCGGCGTCCG32460.16612825961455788No Hit
GTGAGACCCCCGCTCTGCCCCTTCGCGAATTTCGACGATCGTTGCATTAA32310.16536056895090465No Hit
TGAACAAAACGAAAGGTGAGTAAGACTCCTGGGCTCAGCGCGGTGGAAGG32300.16530938957332778No Hit
GTGAGTCCAACTGCGAATTTCGACGATCGTTGCATTAACTCGCGAAGGGT32250.16505349268544336No Hit
ACTGAAAGCGGGGCAGGAGCTGGAGCCGACAAGGGAACCGGGCCTGAAGG32160.16459287828725142No Hit
ACAGTGTTTTCTTGCCCTTTTCCTCTGAGACAACAGCAGCAAAAATTACA31730.16239216505144552No Hit
GATTGACCGTGGGCGCCGGGTCTCACTCACCCGCGGACGTCCATGGGGTC30600.15660889538525788No Hit
TGTGGAAGCAAGGAGGCCAAGGACCTCAAGGCACGGAGGAAGAAGTCCCA30460.15589238409918152No Hit
TTGCTATCCATGGCACGAAGTAGACCAGCGGCATCTCGGCCCCACCCTGG29470.15082562571907024No Hit
GCAAAAGCATGTCGCTGAAAAACAAGGCATTGGACTGGGCGATACCACAG29150.14918788563661006No Hit
TCAGTCTTGGTGCCTGTGCCAGCTTCTGCTCCCCCTTCAGGCCCGGTTCC29120.1490343475038794No Hit
CGAGAGATCTGTACGCGCGTGGCTCCTCATTCCTCTTCCTTGGCTTCCCA28970.1482666568402262No Hit
TGCTGTAGGGGTGAGGGAGGCCACTGAGCTCCCGCTGCCTCCCCCGCTCC28860.14770368368688047No Hit
GCGAATTTCGACGATCGTTGCATTAACTCGCGAAGGGTCAAATATCGTAT28350.14509353543045952No Hit
TTGTCCTCCCGGATGACCATGTTGTAGCTGCTGCTGGCCACCACAAAGAT27840.14248338717403855No Hit
ATGAAATCTCACGAACCCAAAGAGGCGGCTGTGGAGACAGACACAGTTTA27810.1423298490413079No Hit
TGGTCACAAATAATTTCTCCCCCACAGAAACCCAGTATTTTCAAGTGGTG25250.1292279283816262No Hit
ATCTGGTGATTGGGGGCAGTCCCTGCAATGACCTCTCCATCGTCAACCCT24960.12774372643189663No Hit
TTGTACTGCTGCTTCCTCTGGTGATGGAACCTTGGGGAGGGTCCCCACGC24740.12661778012520525No Hit
AGAAGTACTCATTATCTGAGGAGCCGGTCACCTGTACCATCTGTAGCTGG24300.12436588751182243No Hit
TTGTCCTCCCGGATGACCATGTTGTAGCTGCTGCTGGCCACCACGAAGAT23580.12068097232628697No Hit
AAAAACAGTCCAGTCGCTAATGCTGGCATTGATAAGGCGGCTTCTTGTTG23510.12032271668324877No Hit
TGCCTCATTTGGAGACAGAAATATAGAGCTTGGTGGAAGGCCGTTCTCGT23390.11970856415232621No Hit
AGCAGGGTTGACGATGGAGAGGTCATTGCAGGGACTGCCCCCAATCACCA23320.11935030850928803No Hit
ATCAGTGCAGTTGAGTTCTTCACCCAGGTGCTCGGGTCCCCATTCCTCAC23120.1183267209577504No Hit
CCATACATGAGACGGCCCAATCCAGTTAGTCCTTATCCAAACTCTTCACA23100.11822436220259665No Hit
CCCTGAAGAAGCGAATTTCGACGATCGTTGCATTAACTCGCGAAGGGTCA23080.11812200344744286No Hit
GATGCCAGCATGGCACCCCTTGGCTTGCAGGTGCCCATCAGAAGCGACTG22900.11720077465105899No Hit
GATGTTTCATGTTCTCAAAGGGTGCCTACACAAGTTGAGACTCTGTGGTT22770.11653544274255956No Hit
AATGGTAGCCATCTGGTGATGGGACTATGAAGGTCGGGCACAATTCCTGG22010.11264581004671653No Hit
GGTACAAATTCTCAGATCATCAGTCCTCACCTGAGGGACCTTCCGCGGCA21570.11039391743333374No Hit
CTCCTCCCAGAGACCCCAGTTGCAAACCAGACCTCAGGCGGCTCATAGGG21470.10988212365756493No Hit
GGGACCCAGAAGCAGGGGCGGCGTCCGCTCACTTGAGGCCCACGGAGTCC21250.10875617735087353No Hit
GATGTTGAGCATCTTTTCAGGTGCTTAGTGGCCATTTGTCCGTCATCTTT21210.108551459840566No Hit
GACCCTTCGCGAATTTCGACGATCGTTGCATTAACTCGCGAAGGGTCAAA20980.10737433415629771No Hit
GACAGCCACTCGCATTGACCATTCGCGAATTTCGACGATCGTTGCATTAA20950.10722079602356709No Hit
GGTCAATTCTCATTGAGTGTGCAAAGCGTGAGCTGCATGCCACAACCCCT20810.10650428473749074No Hit
TCCTCCTGCCACGACTTCGGGACCTAGAGGAACTTTGGCTCCGGCTGCGG20790.10640192598233697No Hit
AACTCAGCATCAAGCAGGCATTTTATGGGAAGCTTTCTAAACTCCAACTG20110.10292172830710902No Hit
CCACCACCCTTGTGCTCTGCACCCAGGGCATATGCCTGAGCGTTTCGATG19930.10200049951072515No Hit
CTTAGTCTGCCATCTCTTCCTCCGCAGCCTACATGAAGCGGCGGCACAGC19810.10138634697980256No Hit
GTATTGACGTGACATTGGCAACAAGGTAGGGGGTGGAAAGAGAGAAAGAG19710.10087455320403375No Hit
TTGAGGAGCATTGGAATTCCCAGGTCTTTTGAGGGAGATGTGAACTCTGG19690.10077219444888No Hit
CTCGCCCTTTGTCATCTTTCCCGAGATCGTGAGGAATGGGGACCCGAGCA19670.10066983569372621No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGGT18650.0141.501168
ACGGTGA18800.0140.757811
CGGGCTA7450.0140.134058
TATTTGC81850.0139.950961
ATTGCCG22650.0138.91815145
GTTGACG3300.0138.408927
ATAGGGG11850.0137.658071
CCGGACT13900.0137.176097
GGTCGCA5400.0136.944274
AGGTTTC12500.0136.879844
CGGTGAG19450.0136.799322
ATGCGCG46800.0136.789378
ATTTGCC83750.0136.689392
GGCGTGA9050.0136.187673
GGCATCG19400.0136.030765
CGGACTT13950.0135.644998
GCTACGC14350.0135.40053145
GGGTTCT10850.0134.976792
GGTGTAC7050.0134.716165
CATCGGG19650.0134.669057
Produced by FastQC (version 0.11.5)