FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405461

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405461
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1610624
Sequences flagged as poor quality0
Sequence length35-251
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGG8103.6379788E-1252.62428245
GCGTCGG9203.7623977E-837.0658245
AAGCGTC2100.00230851716.136395240-244
GTAGGGG28254.0262134E-513.579858245
AGAGCGG18951.4436191E-613.496253245
TCGGTGG10550.003970072612.121043245
GGGGGCG43451.6722033E-510.791307245
AGCGTCG22004.846588E-79.687652245
GTAGGGT9300.007065219.16681245
TCCTCCC47354.7019168E-49.002253245
GATAGGG24401.274293E-78.734768245
AGGGGGG68803.6039492E-68.673827245
GGATAGG27751.3200406E-87.6803007245
AAAGAGG50550.0084558387.5891395245
GGGGTGG74509.1013026E-57.4380355245
CGGGTAG7950.00136371337.1968594235-239
GTGGGGG67800.00206659086.9156685245
GGGTAGA8350.00229098166.8521235-239
GTAGGAT25401.0550139E-96.7127037245
GAGGGGG69950.0028704846.703107245
Produced by FastQC (version 0.11.5)