FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405462

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405462
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1083337
Sequences flagged as poor quality0
Sequence length35-251
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTCGCA700.00967926248.2828061
GGATCGG4400.045.988068245
ACTCCGA2350.001567099123.9701881
GTCACCG10650.002166859616.285513245
GTTAAGT5050.0079887613.3853321
GGGATCG3655.202346E-712.7077875240-244
AGGATCG4650.0096481512.433026245
GTAGCAT10450.0099825038.62468051
TCGTATG7307.297876E-57.9196677245
CTCGTAT6401.09112734E-47.3346186235-239
TATGCCG4950.0024280897.294726235-239
CTTTATG15550.0040279037.2450086
ATGCCGT6450.00344029536.5919595240-244
ACCGCTC10208.150666E-66.442142240-244
ATAGAAA20650.0091224066.00125272
CCGCTCA11302.4516668E-45.4729705240-244
ATCGGAA8700.00150995815.3272943225-229
TCGTGCC6000.00250914975.25746135-39
Produced by FastQC (version 0.11.5)