FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405464

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405464
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1225163
Sequences flagged as poor quality0
Sequence length35-251
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGTGC4505.430953E-639.359642245
TCGTTAC406.7137583E-427.72105415-19
GGATCGG3602.372144E-824.599775245
GCGAGAA2750.004280629620.1607671
ATGCCGT8058.77612E-518.335236245
TATGCCG6552.7091792E-618.027317245
CCCGATC1350.004703263314.50097220-224
CGGTACA2100.0050732611.304708225-229
GCGGTAC2650.002432144210.238226225-229
GTCACAT24600.0041325899.599913245
GGTCAAT10850.001495479.1977691
CGTATGC10405.92657E-58.515306245
GGCTAAA12650.00599610067.8889961
AGGATCG5555.931983E-47.781733240-244
GGATGCT13200.0087763357.5602881
TTACTCG8353.5547782E-77.5233307240-244
ACTCGAT7951.6832702E-47.4263473245
CTCGTAT8251.5745001E-57.1386147240-244
ATCGGAA11851.0189979E-86.5824876225-229
GGCATTT22004.3442068E-46.5522491
Produced by FastQC (version 0.11.5)