FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405465

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405465
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1225163
Sequences flagged as poor quality0
Sequence length35-251
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGG3701.0450424E-438.05215245
CGAGTAA1300.002690899434.2493821
GCGTCGG6951.2249984E-732.412766245
TCGGGGG10050.001752846516.811098245
GCTACAT8600.002212565910.3544651
TTACCGA5500.00127670299.359321230-234
GGCCAAC13450.00109007488.2758731
AGGCAGG42551.3838791E-47.941318245
GTGGGAT16057.325236E-47.6287561
GCCAAGG37900.0031426257.4297075245
GTTCAAT15300.00386874457.27519561
GTTGCTT20301.5487884E-47.1282581
ATCGGAA12751.9990694E-96.759986235-239
GGGGTGG54303.0572907E-46.741467245
GATAGGG12901.6041547E-46.548509245
GAGGGGG52650.00158628916.417912245
GGCGGGG51800.0094433665.9796233245
GCTGTGT29103.2091848E-45.7376541
TAGGATA9905.0515533E-55.721265225-229
GAGAGCG9459.86947E-45.6607637240-244
Produced by FastQC (version 0.11.5)