FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405466

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405466
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1408555
Sequences flagged as poor quality0
Sequence length35-251
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGATCG5850.001161306325.232397245
TATGCCG6251.1710046E-518.894018245
GCCTAAC3609.536988E-418.4125461
TCCGGTC3050.00795374518.11077
GTATGCC11900.003947308814.884993245
ATGCCGT8309.517587E-614.227424245
TGAACGC6500.003462154711.8973383
CTCGTAT7852.3517304E-411.282257245
TCGTATG9557.407802E-49.273895245
GTTAGCT10150.0092357438.7074111
GGGGATT12600.00598295137.89109131
GGTGCTT24002.5058735E-56.9047051
GTTGAAT24102.6557273E-56.8760551
GACCTTA3550.00231233826.846355415-19
GTTTTAC23301.2675073E-46.63799951
GGTCAAA16700.0097253926.6152861
GGTGTCC18400.0033692246.60450031
GCTGGGA58950.06.55917641
GGTTGAT18750.00410359776.4812161
GGGATTA24101.9663293E-46.4176514
Produced by FastQC (version 0.11.5)