FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405467

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405467
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1408555
Sequences flagged as poor quality0
Sequence length35-251
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGG4553.4197E-948.639935245
TCGGCGG3950.00718959828.01414245
GCGTCGG7903.916869E-421.010605245
ATCGTTT4700.005359861514.2172929
GTAGGGG20851.3440424E-411.941278245
GGATAGG21800.002603275710.151913245
GTAACAG13158.673122E-48.4691091
GCGTCGT16857.632116E-88.208891245
GGTGACC13450.009981397.4521861
GTGAACT19708.4964064E-46.7838851
TTTGGAC20400.00126260916.5511056
TATATTT48200.00538175746.3133526245
GGTGCTT24902.7039886E-46.26169871
GCTCAGA19750.0064829036.20281841
GTTTGGA32455.680504E-66.1776211
GTAGGGT9201.4595082E-46.0801334240-244
GGGGAAC22000.00295284726.0746611
GGTGGAT27851.7532405E-45.99831871
TAGATCG8005.271333E-45.9460425230-234
AAAGCGT7150.0041181655.896963235-239
Produced by FastQC (version 0.11.5)