FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405469

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405469
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1346715
Sequences flagged as poor quality0
Sequence length37-251
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCGCC1400.006958863752.484303245
GATCGGG3401.4442709E-436.018635245
CGTAGGG600.00896249726.78056240-244
GCGTCGG8050.001009379418.255407245
AGAGCGG13201.0648686E-414.844045245
CGAGTCA1356.7909656E-414.770657220-224
CGATCGG2206.7451136E-411.937494240-244
GTATAAC9200.002659013310.1278862
TAGCGGA2050.00558494039.258838145-149
GAGGGGG62601.1586963E-98.216392245
GGATTAC22854.9089023E-57.13605985
GAAGGGG38050.00161526397.080667245
GTCAAAT22853.7892783E-46.62634131
TGGAGGG44900.00127084096.545926245
TTACAGG34908.942481E-66.00708448
GTGGAAC29552.1355359E-45.9122181
GATTACA26550.00231001745.70289666
GTGGGGG56150.00246490895.67061245
GCCCCCC51850.0063604785.668506245
GGCGGGT7900.00142910415.497922235-239
Produced by FastQC (version 0.11.5)