FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405470

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405470
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1104939
Sequences flagged as poor quality0
Sequence length35-251
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGAACC350.0024360989207.22348245
TGATCGG709.32356E-5155.4176245
CGATCGG1003.8576196E-4108.792336245
CGCATAA1400.00930449748.764046240-244
GTCGGCT2002.654378E-531.2277032
TCGTATG11101.906301E-919.60222245
GCTCGGG4704.698771E-415.5031151
CGTCTTC14450.003651374315.057761245
GGTCGGC5108.7299844E-414.2871851
GGATCGG2750.003024508713.186949245
CGTATGC11103.6379788E-1213.068148245
CTCGGGC6654.505187E-412.5223872
ATGCCGT9551.3666109E-511.391867245
GGTGTAC7800.001715097610.6761371
TAGGGTA6950.00887572710.4841217
CTCGTAT10409.276932E-510.4608245
GGTGTTC8400.00317392969.9135564
GCTAGAA11404.0958117E-49.1309071
GTCAGCA10500.00197233738.92221
TATGCCG8351.1153454E-58.686015245
Produced by FastQC (version 0.11.5)